Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion.
نویسندگان
چکیده
منابع مشابه
Importance of Campylobacter jejuni FliS and FliW in Flagella Biogenesis and Flagellin Secretion
Flagella-driven motility enables bacteria to reach their favorable niche within the host. The human foodborne pathogen Campylobacter jejuni produces two heavily glycosylated structural flagellins (FlaA and FlaB) that form the flagellar filament. It also encodes the non-structural FlaC flagellin which is secreted through the flagellum and has been implicated in host cell invasion. The mechanisms...
متن کاملThe Minor Flagellin of Campylobacter jejuni (FlaB) Confers Defensive Properties against Bacteriophage Infection
A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Inactivation of the flaB gene resulted in an increase in the susceptibility of PT14 cultures to infection by CP_F1 and an increase in bacteriophage yi...
متن کاملStructural and functional analysis of two Campylobacter jejuni flagellin genes.
The purpose of this study was to characterize the flagellin gene of Campylobacter jejuni and to study the structure of this protein and the regulation of its synthesis. A part of the flagellin gene of C. jejuni strain 81116 was recently cloned by us. This DNA fragment was used as a probe to isolate the other homologous flagellin sequences from genomic libraries. The flagellin nucleotide sequenc...
متن کاملDistribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni.
The complex flagellar filaments of the LIO8 serogroup member Campylobacter coli VC167 are composed of two highly related subunit proteins encoded by the flaA and flaB genes which share 92% identity. Using oligonucleotide primers based on the known DNA sequence of both the flaA and flaB genes from C. coli VC167 in the polymerase chain reaction, we have shown conservation of both fla genes among ...
متن کاملRisk factors for infection with Campylobacter jejuni flaA genotypes.
We aimed to explore Campylobacter genotype-specific risk factors in Australia. Isolates collected prospectively from cases recruited into a case-control study were genotyped using flaA restriction fragment-length polymorphism typing (flaA genotyping). Exposure information for cases and controls was collected by telephone interview. Risk factors were examined for major flaA genotypes using logis...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1991
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1991.tb07736.x